Recombinant Mouse CCL27/CTACK Protein

SDS-Page: Mouse CCL27/CTACK Protein [NBP2-35164]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CCL27/CTACK Protein Summary

• Description: A single, non-glycosylated polypeptide chain containing 95 amino acids corresponding to CCL27/CTACK Source: E. coli

  Uniprot ID: Q9Z1X0

  Amino Acid Sequence: LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN

• Details of Functionality: CCL27 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 10-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL27
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL27/CTACK as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 10.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, with 5% trehalose.
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CCL27/CTACK Protein

• ALP
• C-C motif chemokine 27
• CCL27
• chemokine (C-C motif) ligand 27
• CTACK
• CTACK/CCL27
• CTACKSmall-inducible cytokine A27
• CTAK
• cutaneous T-cell attracting chemokine
• Cutaneous T-cell-attracting chemokine
• ESKINE
• IL-11 Ralpha-locus chemokine
• ILC
• ILCCC chemokine ILC
• PESKY
• SCYA27IL-11 R-alpha-locus chemokine
• skinkine
• small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also named IL-11 R-alpha-locus chemokine (ILC), skinkine, eskine and cutaneous T-cell-attracting chemokine (CTACK), is a small cytokine belonging to the CC chemokine family. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin and it elicits its chemotactic effects by binding to the chemokine receptor CCR10. CCL27 can attract skin-associated memory T-lymphocytes. Studies showed it may play a role in mediating homing of lymphocytes to cutaneous sites and cell migration during embryogenesis. Mature mouse CCL27 is a 95 amino acid (a.a.) protein that shares 57 % a.a. and 87 % a.a. sequence identity with human and rat CCL27, respectively. It shares 18 %~31 % a.a. sequence identity with other mouse CC chemokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL27