CCL27/CTACK Antibody [Alexa Fluor™ Plus 405]

• Reactivity: Mu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 405

CCL27/CTACK Antibody [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects mouse CCL27/CTACK in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL27/CTACK Antibody [Alexa Fluor™ Plus 405]

• ALP
• C-C motif chemokine 27
• CCL27
• chemokine (C-C motif) ligand 27
• CTACK
• CTACKSmall-inducible cytokine A27
• CTAK
• cutaneous T-cell attracting chemokine
• Cutaneous T-cell-attracting chemokine
• ESKINE
• IL-11 Ralpha-locus chemokine
• ILC
• ILCCC chemokine ILC
• PESKY
• SCYA27IL-11 R-alpha-locus chemokine
• skinkine
• small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively (2). It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4-7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10-12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA⁺ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13-15).

1. Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
2. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 258:737.
3. Gortz, A. et al. (2002) J. Immunol. 169:1387.
4. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. USA 96:14470.
5. Homey, B. et al. (2000) J. Immunol. 164:3465.
6. Homey, B. et al. (2002) Nat. Med. 8:157.
7. Inokuma, D. et al. (2006) Stem Cells 24:2810.
8. Jarmin, D. et al. (2000) J. Immunol. 164:3460.
9. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
10. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
11. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
12. Soler, D. et al. (2003) Blood 101:1677.
13. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
14. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
15. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.