CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11) - Azide and BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
61016.11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
0.5 mg/ml

CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11) - Azide and BSA Free Summary

Immunogen
Purified recombinant human eotaxin 2, expressed in E. coli as immunogen.
Localization
Secreted
Specificity
When used as a capture in human eotaxin 2 sandwich ELISAs, this exhibits less than 0.03 % cross-reactivity with recombinant human eotaxin, MIP1 alpha, and MCP3.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL24
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: Use at a concentration of 2 ug/ml. Neut: Use at a concentration of 1 - 5 ug/ml. WB: Use at a concentration of 1 - 2 ug/ml. Predicted molecular weight: 10.6 kDa.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11) - Azide and BSA Free

  • CCL24
  • chemokine (C-C motif) ligand 24
  • Ckb-6
  • Eosinophil chemotactic protein 2
  • Eotaxin-2
  • member 24
  • MPIF2
  • MPIF-2
  • MPIF2Myeloid progenitor inhibitory factor 2
  • MPIF-2Small-inducible cytokine A24
  • SCYA24CK-beta-6

Background

Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CCL24
Entrez