Human CCL22/MDC ELISA Kit (Colorimetric) Summary
Description |
CCL22 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human CCL22. |
Standard Curve Range |
31.2 to 2000 pg/mL |
Sensitivity |
1 pg/mL |
Assay Type |
Sandwich ELISA |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
CCL22 |
Applications/Dilutions
Dilutions |
|
Application Notes |
Useful for quantification. |
Publications |
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Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Kit Components
Components
|
- 96-well plate precoated with anti- human MDC antibody
- ABC diluent buffer
- Antibody diluent buffer
- Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
- Biotinylated anti- human MDC antibody, dilution 1:100
- Lyophilized recombinant human MDC standard
- Sample diluent buffer
- TMB color developing agent
- TMB stop solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human CCL22/MDC ELISA Kit (Colorimetric)
Background
Chemokines are a large family of small secreted proteins that regulate migration of white blood cells. Based on the arrangement of the first two of the four conserved cysteine residues, chemokines are classified into four subfamilies, CXC, CC, C and recently identified CX3C (1). The CC chemokines, in which the first 2 cysteines are adjacent, usually act on monocytes, T lymphocytes, and, in some cases, eosinophils, basophils, or mast cells. The CC subfamily proteins are 70 to 100 amino acids long, have 25 to 75% identity with each other, and include novel member of the CC chemokine subfamily, designated ABCD1 (SCYA22). The SCYA22 protein shares 28 to 34% identity with other CC chemokines and contains the characteristic 4-cysteine motif and 9 other highly conserved residues (2). The activated T cell-attracting CC chemokine CCL22 is expressed by stimulated B cells and mature dendritic cells (DC). Both identical and distinct proteins contribute to expression of CCL22 in DC and B cells (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for CCL22/MDC ELISA Kit (KA0386)(2)
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Product General Protocols
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FAQs for CCL22/MDC ELISA Kit (KA0386). (Showing 1 - 1 of 1 FAQs).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.