CCL22/MDC Antibody (158113) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB
• Clone: 158113
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

CCL22/MDC Antibody (158113) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant mouse CCL22/MDC
  Gly25-Ser92
  Accession # O88430
• Specificity: Detects mouse CCL22/MDC in direct ELISAs and Western blots. Shows 12% cross-reactivity with recombinant human (rh) CCL22 and recombinant mouse (rm) MIP-3 alpha , 25% cross-reactivity with rmCTACK, 6% cross-reactivity with rhBLC/BCA-1, and no cross-rea
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL22/MDC Antibody (158113) [Alexa Fluor® 532]

• A-152E5.1
• ABCD-1
• CC chemokine STCP-1
• C-C motif chemokine 22
• CCL22
• chemokine (C-C motif) ligand 22
• DC/B-CK
• Macrophage-derived chemokine
• MDC
• MDCStimulated T-cell chemotactic protein 1
• MGC34554
• SCYA22MDC(1-69)
• small inducible cytokine A22
• small inducible cytokine subfamily A (Cys-Cys), member 22
• Small-inducible cytokine A22
• STCP-1
• stimulated T cell chemotactic protein 1

Background

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.