CCL2/MCP1 Antibody (ECE.2)

Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.

• Reactivity: Mu
• Applications: WB, ELISA, IHC
• Clone: ECE.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

CCL2/MCP1 Antibody (ECE.2) Summary

• Immunogen: Murine CCL2/MCP1
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Western Blot 1:100-1:2000
• Application Notes: The antibody is useful for detection of MCP-1 by Western blot. Furthermore it can be used in immunoassays. The typical starting working dilution is 1:10. It is recommended that users test the reagent and determine their own optimal dilutions. Although not tested this antibody may be useful in immunohistochemistry on frozen and paraffin sections. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26413869)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CCL2/MCP1 Antibody (ECE.2)

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine-beta family of cytokines. The protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. It may play a inprotant role in the initiation and/or progression of pulmonary hypertension (PH). Blockade of a systemic MCP-1 signal pathway in vivo may prevent PH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL2/MCP1 Antibody (NBP1-42312)(5)

Publications using NBP1-42312

• An L, Kim D, Donahue L et al. Sexual dimorphism in melanocyte stem cell behavior reveals combinational therapeutic strategies for cutaneous repigmentation bioRxiv 2023-05-23 [PMID: 37293072] (IHC-P, Mouse)
• Makabe K, Sugita S, Mandai M et al. Microglia dynamics in retinitis pigmentosa model: formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration Scientific reports 2020-09-07 [PMID: 32895435] (IF/IHC, Mouse)
• Pena CG, Nakada Y, Saatcioglu HD et al. LKB1 loss promotes endometrial cancer progression via CCL2-dependent macrophage recruitment. J Clin Invest 2015-11-02 [PMID: 26413869] (WB)
• Zhu X, Westcott MM, Bi X et al. Myeloid Cell-Specific ABCA1 Deletion Protects Mice From Bacterial Infection Circ Res 2012-11-09 [PMID: 22955730] (IF/IHC, Mouse)
• Kajii M, Suzuki C, Kashihara J et al. Prevention of excessive collagen accumulation by human intravenous immunoglobulin treatment in a murine model of bleomycin-induced scleroderma Clin Exp Immunol 2011-02-01 [PMID: 21091669] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: CCL2