CCL14/HCC-1/HCC-3 Antibody (256413)

• Reactivity: Hu
• Applications: WB
• Clone: 256413
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL14/HCC-1/HCC-3 Antibody (256413) Summary

• Immunogen: E. coli-derived recombinant human CCL14/HCC‑1/HCC‑3
  Thr20-Asn109
  Accession # NP_116738
• Specificity: Detects human CCL14/HCC‑1/HCC‑3 in direct ELISAs and Western blots. Detects both CCL14a and CCL14b. In Western blots, no cross‑reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL2, 3, 4, 6, 7, 9/10/MIP-1 gamma , 11, 12, 19, 20, 21, 22, 24, 25, 28, or recombinant rat CCL20 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL14/HCC-1/HCC-3 Antibody (256413)

• C-C motif chemokine 14
• CCL14
• chemokine (C-C motif) ligand 14
• Chemokine CC-1/CC-3
• chemokine CC-3
• CKB1
• FLJ16015
• HCC-1
• HCC-1(1-74)
• HCC-1/HCC-3
• HCC-3
• hemofiltrate CC chemokine 1
• MCIF
• member 14
• NCC2CC-1
• NCC-2CKb1
• new CC chemokine 2
• SCYA14CC-3
• Small-inducible cytokine A14

Background

CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20‑93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28‑93) allows potent signaling through CCR1 and CCR5. CCL14b, also called HCC-3, is a 90 amino acid peptide (aa 20‑109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL14
• Uniprot:
  • NP_116738()