CCL14/HCC-1/HCC-3 Antibody (256409)

Recombinant Human CCL14a/HCC‑1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCL14/HCC‑1/HCC‑3 Monoclonal Antibody (Catalog # MAB3241). The ND50 is typically 0.5‑2.5 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 256409
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL14/HCC-1/HCC-3 Antibody (256409) Summary

• Immunogen: E. coli-derived recombinant human CCL14/HCC‑1/HCC‑3
  Thr20-Asn109
  Accession # NP_116738
• Specificity: Detects human CCL14/HCC-1/HCC-3 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 6, 7, 9, 11, 12, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CCL14a/HCC-1 Biotinylated Antibody (Catalog number BAF324) Standard: Recombinant Human CCL14a/HCC-1 aa 20-93 (Catalog number 324-HC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL14/HCC-1/HCC-3 Antibody (256409)

• C-C motif chemokine 14
• CCL14
• chemokine (C-C motif) ligand 14
• Chemokine CC-1/CC-3
• chemokine CC-3
• CKB1
• FLJ16015
• HCC-1
• HCC-1(1-74)
• HCC-1/HCC-3
• HCC-3
• hemofiltrate CC chemokine 1
• MCIF
• member 14
• NCC2CC-1
• NCC-2CKb1
• new CC chemokine 2
• SCYA14CC-3
• Small-inducible cytokine A14

Background

CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20-93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28-93) allows potent signaling through CCR1 and CCR5. CCL14b, also known as HCC-3, is a 90 amino acid peptide (aa 20-109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL14/HCC-1/HCC-3 Antibody (MAB3241)(2)

Publications using MAB3241

• J Zhang, H Zhang, X Ding, J Hu, Y Li, J Zhang, H Wang, S Qi, A Xie, J Shi, M Xiang, Y Bin, G Wang, L Wang, Z Wang Crosstalk between macrophage-derived PGE2 and tumor UHRF1 drives hepatocellular carcinoma progression Theranostics, 2022-05-01;12(8):3776-3793. 2022-05-01 [PMID: 35664070] (Neutralization, Western Blot, Human)
• Yi Li, Yuhuan Zheng, Tianshu Li, Qiang Wang, Jianfei Qian, Yong Lu et al. Chemokines CCL2, 3, 14 stimulate macrophage bone marrow homing, proliferation, and polarization in multiple myeloma Oncotarget 2015-09-15 [PMID: 26155942]

Review for CCL14/HCC-1/HCC-3 Antibody (MAB3241) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Ruhul Choudhury B/N Rat 01/20/2017

Summary

• Application: Block/Neutralize
• Sample Tested: uterine endothelial cells conditioned medium
• Species: Rat
• Lot: KZD051511A

Bioinformatics

• Gene Symbol: CCL14
• Uniprot:
  • NP_116738()