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CCL13/MCP-4 Overexpression Lysate

Images

 
Western Blot: MCP4 Overexpression Lysate (Adult Normal) [NBL1-08843] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCP4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CCL13/MCP-4 Overexpression Lysate Summary

Description

CCL13/MCP-4 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC203789

Accession#: NM_005408

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CCL13

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
9.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CCL13/MCP-4 Overexpression Lysate

  • C-C motif chemokine 13
  • CCL13
  • chemokine (C-C motif) ligand 13
  • CKb10
  • MCP4
  • MCP-4
  • MCP-4Monocyte chemoattractant protein 4
  • MGC17134
  • NCC-1Monocyte chemotactic protein 4
  • NCC1Small-inducible cytokine A13
  • new CC chemokine 1
  • SCYA13CK-beta-10
  • SCYL1
  • small inducible cytokine subfamily A (Cys-Cys), member 13

Background

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CCL13/MCP-4 Lysate (NBL1-08843) (0)

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Video Protocols

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Bioinformatics

Gene Symbol CCL13