CCL13/MCP-4 Antibody (73506) [Unconjugated]

Recombinant Human CCL13/MCP‑4 (Catalog # 327‑P4) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP‑4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human CCL13/MCP‑4 Monoclonal Antibody (Catalog # MAB327). The ND50 is typically 10‑30 µg/mL.

• Reactivity: Hu
• Applications: B/N, ELISA(Cap)
• Clone: 73506
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL13/MCP-4 Antibody (73506) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL13/MCP-4
  Gln24-Thr98
  Accession # Q99616.1
• Specificity: Detects human CCL13/MCP-4 in ELISAs. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) CCL2 is observed and no significant cross-reactivity or interference with rhCCL5, 7, 8, 11, 24, recombinant mouse CCL2, 5, 7, 11 or 12 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL13
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 10-30 ug/mL
• Application Notes: ELISA Detection: Human CCL13/MCP-4 Biotinylated Antibody (Catalog number BAF327) Standard: Recombinant Human CCL13/MCP-4 (Catalog number 327-P4)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL13/MCP-4 Antibody (73506) [Unconjugated]

• C-C motif chemokine 13
• CCL13
• chemokine (C-C motif) ligand 13
• CKb10
• MCP4
• MCP-4
• MCP-4Monocyte chemoattractant protein 4
• MGC17134
• NCC-1Monocyte chemotactic protein 4
• NCC1Small-inducible cytokine A13
• new CC chemokine 1
• SCYA13CK-beta-10
• SCYL1
• small inducible cytokine subfamily A (Cys-Cys), member 13

Background

CCL13, also known as Monocyte Chemoattractant Protein-4 (MCP-4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR-2 and CCR-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL13/MCP-4 Antibody (MAB327)(2)

Publications using MAB327

• Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 2020-08-01 [PMID: 32645325]
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-06-16;120(2):220-31. 2006-06-16 [PMID: 16782408] (ELISA Development, Neutralization, Human)

Bioinformatics

• Gene Symbol: CCL13
• Uniprot:
  • Q99616.1()