CCK-AR Antibody

Immunohistochemistry-Paraffin: CCK-AR Antibody [NB100-2805] - Staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Rt
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CCK-AR Antibody Summary

• Immunogen: Peptide with sequence C-FEASQKKSAKERKPST corresponding to internal region according to NP_000721.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCKAR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 2-4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA 1:28000
• Application Notes: ICC/IF successful usage for RIN-14B cells is reported in scientific literature (PMID: 19959964).
• Control:

  CCK-AR Overexpression Lysate

Reactivity Notes

Rat reactivity reported in (PMID: 19959964).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCK-AR Antibody

• CCK1R
• CCK1-R
• CCK-A R
• CCK-A receptor
• CCK-A
• CCKAR
• CCK-AR
• CCKRA
• cholecystokinin A receptor
• cholecystokinin receptor type A
• cholecystokinin type-A receptor
• Cholecystokinin-1 receptor

Background

CCKAR is a receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCK-AR Antibody (NB100-2805)(3)

Publications using NB100-2805

• Bo Bai, Xiaoyu Chen, Rumin Zhang, Xin Wang, Yunlu Jiang, Dandan Li, Zhengwen Wang, Jing Chen Dual-agonist occupancy of orexin receptor 1 and cholecystokinin A receptor heterodimers decreases G-protein-dependent signaling and migration in the human colon cancer cell line HT-29. Biochimica et biophysica acta. Molecular cell research 2017-10-24 [PMID: 28288880]
• El-Kouhen K, Morisset J. Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. Pancreas 2010-03-01 [PMID: 19959964]
• Beglinger C, Degen L, Matzinger D et al. Loxiglumide, a CCK-A receptor antagonist, stimulates calorie intake and hunger feelings in humans. Am J Physiol Regul Integr Comp Physiol 2001-04-01 [PMID: 11247838]

Bioinformatics

• Gene Symbol: CCKAR
• Entrez:
  • 886(Human)
  • 24889(Rat)
• Uniprot:
  • P32238()