CCDC5 Antibody - BSA Free

Western Blot: CCDC5 Antibody [NBP1-88159] - Analysis using Anti-HAUS1 antibody NBP1-88159 (A) shows similar pattern to independent antibody NBP1-88158 (B).

Immunocytochemistry/Immunofluorescence: CCDC5 Antibody [NBP1-88159] - Staining of human cell line U-2 OS shows localization to cytosol & centrosome. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CCDC5 Antibody [NBP1-88159] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CCDC5 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QEERETQVAAWLKKIFGDHPIPQYEVNPRTTEILHHLSERNRVRDRDVYLVIEDLKQKASEYESEAKYLQDLLMESVNFSPANLSSTGSRYLNALVD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HAUS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CCDC5 Protein (NBP1-88159PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CCDC5 Antibody - BSA Free

• CCDC5Enhancer of invasion-cluster
• coiled-coil domain containing 5 (spindle associated)
• FLJ40084
• HAUS augmin-like complex subunit 1
• HAUS augmin-like complex, subunit 1
• HEI-CCoiled-coil domain-containing protein 5
• HEICFLJ21094
• HsT1461

Background

CCDC5 belongs to the HAUS1 family. More specifically, CCDC5 is a component of the HAUS augmen complex. Originally founded in Drosophila, the complex plays an important role in spindle assembly, maintenance of centrosome integrity and completion of cytokinesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAUS1