Nexilin Antibody - Azide and BSA Free

Western Blot: Nexilin Antibody [NBP2-93780] - Analysis of extracts of various cell lines, using Nexilin at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: Nexilin Antibody [NBP2-93780] - Analysis of U-2 OS cells using Nexilin at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Nexilin Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Nexilin Antibody - Azide and BSA Free (NBP2-93780) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 516-675 of human NEXN (NP_653174.3). RFEQMAKAREEEEQRRIEEQKLLRMQFEQREIDAALQKKREEEEEEEGSIMNGSTAEDEEQTRSGAPWFKKPLKNTSVVDSEPVRFTVKVTGEPKPEITWWFEGEILQDGEDYQYIERGETYCLYLPETFPEDGGEYMCKAVNNKGSAASTCILTIESKN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEXN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Nexilin Antibody - Azide and BSA Free

• CMD1CC
• F-actin-binding protein
• MGC104234
• MGC138865
• MGC138866
• Nelin
• NELINnexilin
• nexilin (F actin binding protein)

Background

Nexilin is an F actin binding protein that is involved in regulating cell migration through association with the actin cytoskeleton. It is abundantly expressed in heart and skeletal muscle, and at lower levels in placenta, lung, liver and pancreas. It is also expressed in HeLa, S3 and Molt 4 cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: NEXN