Cbx8 Antibody

Western Blot: Cbx8 Antibody [NB100-96915] - (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cbx8 Antibody Summary

• Description: Novus Biologicals Goat Cbx8 Antibody (NB100-96915) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cbx8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RERGTSRVDDKPSS corresponding to internal region according to NP_065700.1.
• Epitope: RERGTSRVDDKPSS
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CBX8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in K562 lysates (calculated MW of 43.4 kDa band according to NP_065700.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Cbx8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cbx8 Antibody

• chromobox homolog 8 (Drosophila Pc class)
• chromobox homolog 8 (Pc class homolog, Drosophila)
• chromobox homolog 8
• HPC3
• Pc3
• PC3chromobox protein homolog 8
• Polycomb 3 homolog
• polycomb 3
• RC1Pc class 3 homolog
• Rectachrome 1

Background

CBX8 is an essential component of one of the polycomb repressive complexes, which directly regulate the expression of numerous target genes, including the INK4A-ARF locus, involved in cell-fate decisions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cbx8 Antibody (NB100-96915)(1)

Publication using NB100-96915

• Dietrich N, Bracken AP, Trinh E et al. Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus. EMBO J 2007-03-21 [PMID: 17332741]

Bioinformatics

• Gene Symbol: CBX8
• Entrez:
  • 57332(Human)
  • 30951(Mouse)
• Uniprot:
  • NP_065700.1(Human)