Cbx8 Antibody

Images

 
Western Blot: Cbx8 Antibody [NB100-96915] - (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Cbx8 Antibody Summary

Immunogen
Peptide with sequence C-RERGTSRVDDKPSS corresponding to internal region according to NP_065700.1.
Epitope
RERGTSRVDDKPSS
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CBX8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 40 kDa band observed in K562 lysates (calculated MW of 43.4 kDa band according to NP_065700.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Positive Control
Cbx8 Lysate (NBL1-08753)
Publications
Read Publication using NB100-96915.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cbx8 Antibody

  • chromobox homolog 8 (Drosophila Pc class)
  • chromobox homolog 8 (Pc class homolog, Drosophila)
  • chromobox homolog 8
  • HPC3
  • Pc3
  • PC3chromobox protein homolog 8
  • Polycomb 3 homolog
  • polycomb 3
  • RC1Pc class 3 homolog
  • Rectachrome 1

Background

CBX8 is an essential component of one of the polycomb repressive complexes, which directly regulate the expression of numerous target genes, including the INK4A-ARF locus, involved in cell-fate decisions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cbx8 Antibody (NB100-96915)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CBX8
Entrez
Uniprot