CBX1 Antibody

Western Blot: CBX1 Antibody [NB100-2423] - (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: CBX1 Antibody [NB100-2423] - 2.5ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CBX1 Antibody Summary

• Description: Novus Biologicals Goat CBX1 Antibody (NB100-2423) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CBX1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QSQKTAHETDKSE corresponding to internal region according to NP_001120700.1.
• Specificity: Variants (NP_006798.1; NP_001120700.1) encode the same protein.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CBX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 10 ug/ml
• Application Notes: WB: Approx. 25 kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25 kDa band according to NP_006798.1). IHC-P: Human placenta shows strong nuclear staining in trophoblasts.
• Control:

  A-431 Whole Cell Lysate
  CBX1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBX1 Antibody

• CBXM31HP1-BETA
• chromobox homolog 1 (Drosophila HP1 beta)
• chromobox homolog 1 (HP1 beta homolog Drosophila )
• chromobox homolog 1
• chromobox protein homolog 1
• Heterochromatin protein 1 homolog beta
• heterochromatin protein 1-beta
• heterochromatin protein p25 beta
• Heterochromatin protein p25
• HP1 beta homolog
• HP1 beta
• HP1Hsbeta
• HP1Hs-beta
• MOD1
• Modifier 1 protein
• p25beta

Background

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBX1 Antibody (NB100-2423)(1)

Publication using NB100-2423

• Sharma GG, Hwang KK, Pandita RK et al. Human heterochromatin protein 1 isoforms HP1(Hsalpha) and HP1(Hsbeta) interfere with hTERT-telomere interactions and correlate with changes in cell growth and response to ionizing radiation. Mol Cell Biol 2003-11-01 [PMID: 14585993]

Bioinformatics

• Gene Symbol: CBX1
• Entrez:
  • 10951(Human)
  • 12412(Mouse)
  • 360609(Rat)
• Uniprot:
  • NP_006798.1(Human)