CBR1 Antibody

Western Blot: CBR1 Antibody [NB100-1066] - Staining of Human Liver lysate (35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: CBR1 Antibody [NB100-1066] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: CBR1 Antibody [NB100-1066] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CBR1 Antibody Summary

• Description: Novus Biologicals Goat CBR1 Antibody (NB100-1066) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HGQFVSEKRVEQW corresponding to C-Terminus according to NP_001748.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CBR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.03 - 0.1 ug/mL
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBR1 Antibody

• 15-hydroxyprostaglandin dehydrogenase [NADP+]
• carbonyl reductase (NADPH) 1
• carbonyl reductase (NADPH) 1, EC 1.1.1.18410EC 1.1.1.197,15-hydroxyprostaglandin dehydrogenase
• carbonyl reductase [NADPH] 1
• carbonyl reductase 1
• CBR
• CRN
• EC 1.1.1.184
• EC 1.1.1.189
• hCBR1
• NADPH-dependent carbonyl reductase 1
• Prostaglandin 9-ketoreductase
• Prostaglandin-E(2) 9-reductase
• SDR21C1
• short chain dehydrogenase/reductase family 21C, member 1

Background

Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBR1 Antibody (NB100-1066)(2)

Publications using NB100-1066

• Jana B, Ca?ka J, Palus K, Witek K Noradrenaline and adrenoreceptors regulate prostaglandin F2? formation in endometrium after experimentally-induced inflammation in the pig Annals of Animal Science 2023-10-05 (WB, Porcine)
• Jana B, Całka J, Mówińska A Noradrenaline and Adrenoreceptors Promote Prostaglandin F2 alpha Generation in Lipopolysaccharide-Exposed Endometrial Epithelial Cells of Pigs ( Sus scrofa domesticus ). International Journal of Molecular Sciences 2025-06-26 [PMID: 40565335]

Bioinformatics

• Gene Symbol: CBR1
• Entrez:
  • 873(Human)
• Uniprot:
  • NP_001748(Human)