CBR1 Antibody (4E12) - BSA Free

Western Blot: CBR1 Antibody (4E12) [NBP1-74041] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: CBR1 Antibody (4E12) [NBP1-74041] - Analysis of CBR1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-CBR1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CBR1 Antibody (4E12) - BSA Free Summary

• Description: Novus Biologicals Mouse CBR1 Antibody (4E12) - BSA Free (NBP1-74041) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human CBR1 (1-277 aa ) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CBR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CBR1 Antibody (4E12) - BSA Free

• 15-hydroxyprostaglandin dehydrogenase [NADP+]
• carbonyl reductase (NADPH) 1
• carbonyl reductase (NADPH) 1, EC 1.1.1.18410EC 1.1.1.197,15-hydroxyprostaglandin dehydrogenase
• carbonyl reductase [NADPH] 1
• carbonyl reductase 1
• CBR
• CRN
• EC 1.1.1.184
• EC 1.1.1.189
• hCBR1
• NADPH-dependent carbonyl reductase 1
• Prostaglandin 9-ketoreductase
• Prostaglandin-E(2) 9-reductase
• SDR21C1
• short chain dehydrogenase/reductase family 21C, member 1

Background

Carbonyl reductase 1 (CBR1) is a NADPH-dependent, monomeric, and cytosolic enzyme belonging to a family of short-chain dehydrogenases/reductases. This protein consists of 277 amino acid residues and is widely distributed in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBR1
• Uniprot:
  • NP_001748(Human)