CBL Antibody

Western Blot: CBL Antibody [NB100-869] - staining (2 ug/ml) of 3T3 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Mu, Hu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CBL Antibody Summary

• Description: Novus Biologicals Goat CBL Antibody (NB100-869) is a polyclonal antibody validated for use in WB and ELISA. Anti-CBL Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-REFVSISSPAHVAT corresponding to C-Terminus according to NP_005179.2.
• Predicted Species: Human (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CBL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 120 kDa band observed in 3T3 lysate.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBL Antibody

• Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Casitas B-lineage lymphoma proto-oncogene
• CBL
• CBL2
• CBL2E3 ubiquitin-protein ligase CBL
• EC 6.3.2
• EC 6.3.2.-
• oncogene CBL2
• Proto-oncogene c-Cbl
• RING finger protein 55
• RNF55
• RNF55C-CBL
• Signal transduction protein CBL

Background

The proto-oncogene c-CBL was initially identified as the cellular homologue of v-CBL oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. In more recent studies CBL has been shown to be a negative regulator of tyrosine kinase signaling. The ubiquitin ligase activity of CBL leads to the degradation of tyrosine kinases, thus attenuating the signal of receptors. Targets of CBL include activated protein tyrosine kinases belonging to the Src and Syk/Zap-70 families. An additional mechanism to attenuate receptor signaling is thought to be achieved by CBLs interaction with downstream targets of tyrosine kinases, such as PI-3K and Vav.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBL Antibody (NB100-869)(1)

Publication using NB100-869

• Langdon WY, Hartley JW, Klinken SP et al. v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas. Proc Natl Acad Sci U S A 1989-02-01 [PMID: 2784003]

Bioinformatics

• Gene Symbol: CBL
• Entrez:
  • 867(Human)
  • 12402(Mouse)
• Uniprot:
  • NP_005179.(Human)