Cathepsin L Assay Kit (Fluorometric)

Product Discontinued

Cathepsin L Assay Kit (Fluorometric) Summary

• Description: Rapid and convenient fluorometric assay to measure Cathepsin L activity within 1-2 hrs.
  
  Detection method: Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex/Em = 400/505 nm)Applications: Detects alternative proteolytic enzymes such as the lysosomal cathapsin proteases that can initiate or propagate proapoptotic signals.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65306)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CTSL

Applications/Dilutions

Publications

Read Publication using NBP2-54843.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Cathepsin L Assay Kit (Fluorometric)

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-L will cleave the synthetic substrate FR-AFC to release free AFC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Cathepsin L Kit (NBP2-54843)(1)

Publication using NBP2-54843

• Wu CS, Li YC, Peng SL et al. Coffee as a dietary strategy to prevent SARS-CoV-2 infection Cell & bioscience 2023-11-14 [PMID: 37964389]

Bioinformatics

• Gene Symbol: CTSL