Caspase-8 Antibody (84131)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase‑8 Monoclonal Antibody (Catalog # MAB704), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Caspase‑8 at approximately 55 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

• Reactivity: Hu
• Applications: WB
• Clone: 84131
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Caspase-8 Antibody (84131) Summary

• Immunogen: E. coli-derived recombinant human Caspase-8
  aa 234-391
• Specificity: Detects human Caspase-8 precursor in Western blots and a 42 kDa doublet generated during apoptosis.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASP8
• Purity: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
• Control:

  Caspase-8 Knockout HeLa Cell Lysate
  Caspase-8 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-8 Antibody (84131)

• AIS
• androgen receptor
• CASP8
• Caspase8
• Caspase-8
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• Mch5
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Caspase-8 (Cysteine-aspartic acid protease 8/Casp8; also MCH5 and FLICA) is a 28 kDa member of the peptidase C14A family of enzymes. It is known as the initiating caspase for the apoptotic cascade. Caspase-8 acts on procaspases-3, 4, 6, 7, 9 and 10, in addition to c-FLIPL and procaspase-8 itself. Human procaspase-8 alpha 4 is a 58 kDa (predicted), 496 amino acid (aa) protein and contains two N-terminal death domains plus a catalytic site that utilizes His334Gly335 plus Cys377. Following death domain-containing receptor activation, proteolysis generates a 28 kDa heterodimer. This includes a p18 subunit (aa 234-391; or 217-374 of the 8a/standard form) noncovalently linked to a p10 subunit (aa 402-496; or 385-479 of the 8a/standard form). Association with another p18/p10 heterodimer generates active caspase-8. There are two common procaspase-8 isoform variants. One 53 kDa isoform (8b) shows a deletion of aa 103-134, while a second 55 kDa (8a) isoform shows a deletion of aa 103-134 with a 15 aa insertion after Lys183. The p18 and p10 subunits are 68% and 82% aa identical, human to mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-8 Antibody (MAB704)(4)

Publications using MAB704

• L Xu, X Lin, Y Zheng, H Zhou Silencing of heat shock protein 27 increases the radiosensitivity of non?small cell lung carcinoma cells Mol Med Rep, 2019;0(0):. 2019 [PMID: 31115576] (Western Blot, Human)
• Mongiat M, Ligresti G, Marastoni S, Lorenzon E, Doliana R, Colombatti A Regulation of the extrinsic apoptotic pathway by the extracellular matrix glycoprotein EMILIN2. Mol. Cell. Biol., 2007;27(20):7176-87. 2007 [PMID: 17698584] (Western Blot, Human)
• Uchida M, Iwase M, Takaoka S, Yoshiba S, Kondo G, Watanabe H, Ohashi M, Nagumo M, Shintani S Enhanced susceptibility to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in oral squamous cell carcinoma cells treated with phosphatidylinositol 3-kinase inhibitors. Int. J. Oncol., 2007;30(5):1163-71. 2007 [PMID: 17390018] (Western Blot, Human)
• Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells. J. Cell. Sci., 2006;119(0):4247-56. 2006 [PMID: 17003109] (ICC, Human)

Review for Caspase-8 Antibody (MAB704) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 02/25/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23536519
• Species: Human

Blocking

• Blocking Details: See PMID 23536519

Details

• Detection Notes: See PMID 23536519

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23536519

FAQs for Caspase-8 Antibody (MAB704). (Showing 1 - 1 of 1 FAQs).

1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
   • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Bioinformatics

• Gene Symbol: CASP8