Caspase-8 Antibody (1H11)


Western Blot: Caspase-8 Antibody (1H11) [NBP2-22183] - Western blot analysis using Caspase 8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.
Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using Caspase 8 mouse mAb with DAB staining.
Flow Cytometry: Caspase-8 Antibody (1H11) [NBP2-22183] - Flow cytometric analysis of NIH/3T3 cells using Caspase 8 mouse mAb (blue) and negative control (red).
Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using Caspase 8 mouse mAb with DAB staining.
ELISA: Caspase-8 Antibody (1H11) [NBP2-22183] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Caspase-8 Antibody (1H11) Summary

Purified recombinant fragment of human Caspase 8 expressed in E. Coli.
Nucleus, Cytoplasm
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Ascitic fluid
0.03% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
26 kDa

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Caspase-8 Antibody (1H11)

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Dihydrotestosterone receptorHYSP1
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • spinal and bulbar muscular atrophy


This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-8 Antibody (NBP2-22183). (Showing 1 - 1 of 1 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Secondary Antibodies


Isotype Controls

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Blogs on Caspase-8.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CASP8

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