Caspase-12 Antibody - BSA Free

Western Blot: Caspase-12 Antibody [NBP1-76624] - Analysis of Caspase-12 expression in rat heart tissue lysate with Caspase-12 antibody at (A) 0.25 and (B) 0.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Caspase-12 Antibody - BSA Free [NBP1-76624] - Immunofluorescence of Caspase-12 in mouse heart tissue with Caspase-12 antibody at 20 ug/ml.Green: Caspase-12 Antibody (Large) Blue: DAPI staining

Immunohistochemistry: Caspase-12 Antibody [NBP1-76624] - Staining of human heart tissue using caspase-12 antibody (large) at 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: Caspase-12 Antibody - BSA Free [NBP1-76624] - Immunofluorescence of Caspase-12 in Human Heart tissue with Caspase-12 antibody at 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: Caspase-12 Antibody - BSA Free [NBP1-76624] - Immunofluorescence of Caspase-12 in rat heart tissue with Caspase-12 antibody at 20 ug/ml.

Immunohistochemistry: Caspase-12 Antibody - BSA Free [NBP1-76624] - Immunohistochemistry of Caspase-12 in rat heart tissue with Caspase-12 antibody at 2.5 ug/ml.

Immunohistochemistry: Caspase-12 Antibody - BSA Free [NBP1-76624] - Immunohistochemistry of Caspase-12 in mouse heart tissue with Caspase-12 antibody at 2 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Caspase-12 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12. The immunogen is located within amino acids 170 - 220 of Caspase-12. Amino Acid Squence: CNKKVDYLFDRDNADTD
• Specificity: Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP12
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 0.25-2 ug/ml
• Application Notes: Caspase-12 antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jurkat Staurosporine Treated / Untreated Cell Lysate

• Control Peptide:

  Caspase-12 Peptide (NBP1-76624PEP)

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Caspase-12 Antibody - BSA Free

• Apoptosis Related Cysteine Protease
• CASP 12
• CASP12
• CASP12P1
• Caspase 12 pseudogene 1
• Caspase12
• Caspase-12
• UNQ9415

Background

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP12
• Uniprot:
  • CAA73532(Mouse)