CASK Antibody

Western Blot: CASK Antibody [NBP2-76922] - Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.Positive control:Lane1: SiHaLane2: A549

Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-76922] - ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CASK antibody. Counter stained with hematoxylin.

Flow Cytometry: CASK Antibody [NBP2-76922] - Flow cytometric analysis of HT-29 cells with CASK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-76922] - ICC staining CASK in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CASK antibody. Counter stained with hematoxylin.

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CASK antibody. Counter stained with hematoxylin.

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CASK Antibody Summary

• Immunogen: Recombinant protein within human CASK aa 300-500. (SwissProt: O14936 Human; SwissProt: Q62915 Rat)
• Localization: Plasma membrane. Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CASK Antibody

• calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase
• Calcium/calmodulin-dependent serine protein kinase
• CAMGUK
• CMG
• EC 2.7.11
• EC 2.7.11.1
• FGS4CAGH39
• FLJ22219
• hCASK
• LIN2FLJ31914
• MICPCH
• peripheral plasma membrane protein CASK
• Protein lin-2 homolog
• TNRC8
• trinucleotide repeat containing 8

Background

Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASK