Casein Kinase 2 alpha Antibody - BSA Free

Western Blot: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of human CKII alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 (lot NB100-378-2) used for IP at 6 ug/mg lysate. CKII alpha was also immunoprecipitated by a previous lot (lot NB100-378-1) of this antibody. For blotting immunoprecipitated CKII alpha, NB100-378 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Casein Kinase 2 alpha Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 391) of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).
• Predicted Species: Rat (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSNK2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000- 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Casein Kinase 2 alpha Antibody - BSA Free

• Casein Kinase 2 alpha
• casein kinase 2, alpha 1 polypeptide
• casein kinase II alpha 1 subunit
• CK II alpha
• CK2 catalytic subunit alpha
• CK2A1
• CK2A1casein kinase II subunit alpha
• CKII
• CSNK2A1
• EC 2.7.11
• EC 2.7.11.1
• protein kinase CK2

Background

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 2 alpha Antibody (NB100-378)(1)

Publication using NB100-378

• Golkowski M, Lius A, Sapre T et al. Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity Molecular Cell 2023-02-01 [PMID: 36736316] (IP, Human)

Bioinformatics

• Gene Symbol: CSNK2A1
• Entrez:
  • 1457(Human)
• Uniprot:
  • NP_001886.1(Human)