Casein Kinase 1 alpha Antibody (2E2) - BSA Free

Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Analysis of Casein Kinase 1 alpha in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Casein Kinase 1 alpha antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Lane 1.: Jurkat cell lysate. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Casein Kinase 1 alpha Antibody (2E2) - BSA Free Summary

• Description: Novus Biologicals Mouse Casein Kinase 1 alpha Antibody (2E2) - BSA Free (NBP1-30165) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSNK1A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Casein Kinase 1 alpha Antibody (2E2) - BSA Free

• Casein Kinase 1 alpha
• casein kinase 1, alpha 1
• casein kinase I isoform alpha
• CK1CKI-alpha
• CSNK1A1
• down-regulated in lung cancer
• EC 2.7.11.1
• HLCDGP1
• PRO2975

Background

Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, beta, gamma, e and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSNK1A1
• Entrez:
  • 1452(Human)
• Uniprot:
  • NP_001883(Human)