Casein Kinase 1 alpha Antibody (2E2)

Images

 
Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Lane 1.: Jurkat cell lysate. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 ...read more
Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Analysis of Casein Kinase 1 alpha in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ...read more
Western Blot: Casein Kinase 1 alpha Antibody (AT2E2) [NBP1-30165] Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Casein Kinase 1 alpha Antibody (2E2) Summary

Immunogen
Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli
Specificity
This antibody is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Casein Kinase 1 alpha protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CSNK1A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:2000.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Casein Kinase 1 alpha Antibody (2E2)

  • Casein Kinase 1 alpha
  • casein kinase 1, alpha 1
  • casein kinase I isoform alpha
  • CK1CKI-alpha
  • CSNK1A1
  • down-regulated in lung cancer
  • EC 2.7.11.1
  • HLCDGP1
  • PRO2975

Background

Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, beta, gamma, e and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-85630
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85632
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-34270
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP2-32962
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NB100-687
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB110-56916
Species: Hu
Applications: Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-88080
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-16094
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP2-15843
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-19751
Species: Hu
Applications: WB
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-38166
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2727
Species: Hu, Po, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP1-30165
Species: Hu
Applications: WB, ELISA, ICC/IF

Publications for Casein Kinase 1 alpha Antibody (NBP1-30165) (0)

There are no publications for Casein Kinase 1 alpha Antibody (NBP1-30165).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Casein Kinase 1 alpha Antibody (NBP1-30165) (0)

There are no reviews for Casein Kinase 1 alpha Antibody (NBP1-30165). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Casein Kinase 1 alpha Antibody (NBP1-30165) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Casein Kinase 1 alpha Products

Bioinformatics Tool for Casein Kinase 1 alpha Antibody (NBP1-30165)

Discover related pathways, diseases and genes to Casein Kinase 1 alpha Antibody (NBP1-30165). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Casein Kinase 1 alpha Antibody (NBP1-30165)

Discover more about diseases related to Casein Kinase 1 alpha Antibody (NBP1-30165).
 

Pathways for Casein Kinase 1 alpha Antibody (NBP1-30165)

View related products by pathway.

PTMs for Casein Kinase 1 alpha Antibody (NBP1-30165)

Learn more about PTMs related to Casein Kinase 1 alpha Antibody (NBP1-30165).
 

Research Areas for Casein Kinase 1 alpha Antibody (NBP1-30165)

Find related products by research area.

Blogs on Casein Kinase 1 alpha

There are no specific blogs for Casein Kinase 1 alpha, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Casein Kinase 1 alpha Antibody (2E2) and receive a gift card or discount.

Bioinformatics

Gene Symbol CSNK1A1
Entrez
Uniprot