Carm1 Antibody [Alexa Fluor® 594]

• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

Carm1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Carm1
  Lys209-Leu379
  Accession # Q86X55
• Specificity: Detects human Carm1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Carm1 (Coactivator-associated arginine methyltransferase 1; also PRMT4) is a 60-64 kDa member of the Arg N-methyltransferase family of enzymes. It is ubiquitously expressed, and found in the cytoplasm during mitosis, and in the nucleus during the G1, G2 and S phases of the cell cycle. Carm1 binds to nuclear receptor p160 family coactivators. When bound, it methylates DNA-associated histone H3 arginines, allowing for chromatin remodeling and gene activation. It also plays a role in pre-mRNA splicing through its methylation of splicing factors, and regulates the stability of RNA-binding proteins. Human Carm1 is 608 amino acids (aa) in length. It contains one catalytic site between aa 184-394, and a transactivation domain at the C-terminus (aa 499-608). There is one automethylation site at Arg550, and a phosphorylation site at Ser216 that, when utilized, promotes cytosolic localization. Carm1 likely forms homodimers. There are three potential isoform variants. One shows an alternative start site at Met378, a second possesses a 16 aa substitution for aa 369-608, and a third contains a deletion of aa 539-561. Over aa 209-379, human and mouse Carm1 are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.