Recombinant Rat Cardiotrophin-1/CT-1 Protein

SDS-Page: Rat Cardiotrophin-1/CT-1 Protein [NBP2-35292]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat Cardiotrophin-1/CT-1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 203 amino acids corresponding to Cardiotrophin-1/CT-1 Source: E. coli

  Uniprot ID: Q63086

  Amino Acid Sequence: MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA

• Details of Functionality: Cardiotrophin 1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CTF1
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Cardiotrophin-1/CT-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 21.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat Cardiotrophin-1/CT-1 Protein

• cardiophin 1
• cardiotrophin 1
• Cardiotrophin1
• Cardiotrophin-1
• CT-1CT1
• CTF1

Background

Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CTF1