Recombinant Mouse Cardiotrophin-1/CT-1 Protein

SDS-Page: Mouse Cardiotrophin-1/CT-1 Protein [NBP2-35170]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse Cardiotrophin-1/CT-1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 203 amino acids corresponding to Cardiotrophin-1/CT-1 Source: E. coli

  Uniprot ID: Q60753

  Amino Acid Sequence: MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA

• Details of Functionality: Cardiotrophin-1/CT-1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CTF1
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Cardiotrophin-1/CT-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 21.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse Cardiotrophin-1/CT-1 Protein

• cardiophin 1
• cardiotrophin 1
• Cardiotrophin1
• Cardiotrophin-1
• CT-1CT1
• CTF1

Background

Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL6, IL11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. In addition, CT1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Murine CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide. Human and murine CT1 share 80 % a.a. sequence identity and exhibit crossspecies activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CTF1
• Uniprot:
  • Q60753()
  • Q60753()
  • Q60753()