Cardiotrophin-1/CT-1 Antibody [Unconjugated]

Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD) stimulates proliferation in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Cardiotrophin‑1/CT‑1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Cardiotrophin‑1/CT‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF612). The ND50 is typically 0.1-0.3 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cardiotrophin-1/CT-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Cardiotrophin-1/CT-1
  Ser2-Ala201
  Accession # Q16619
• Specificity: Detects human Cardiotrophin‑1/CT‑1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Cardiotrophin-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTF1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.1-0.3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cardiotrophin-1/CT-1 Antibody [Unconjugated]

• cardiophin 1
• cardiotrophin 1
• Cardiotrophin1
• Cardiotrophin-1
• CT-1CT1
• CTF1

Background

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third  alpha receptor subunit has been implicated in the receptor complex.

1. Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
2. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cardiotrophin-1/CT-1 Antibody (AF612)(3)

Publications using AF612

• Robador PA, San Jose G, Rodriguez C, Guadall A, Moreno MU, Beaumont J, Fortuno A, Diez J, Martinez-Gonzalez J, Zalba G HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia. Cardiovasc. Res., 2011-07-19;92(2):247-55. 2011-07-19 [PMID: 21771897] (Western Blot, Human)
• Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes. Cytokine, 2007-07-16;38(3):137-44. 2007-07-16 [PMID: 17637508] (Neutralization, Human)
• Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy. Circulation, 2003-02-18;107(6):798-802. 2003-02-18 [PMID: 12591746] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CTF1
• Uniprot:
  • Q16619()