![Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize2/Carboxypeptidase-E-CPE-Antibody-Western-Blot-NBP2-15699-img0006.jpg "Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase E. The exact sequence is proprietary. |
| Localization | Secreted |
| Predicted Species | Porcine (95%), Chimpanzee (99%), Bovine (94%), Rhesus Macaque (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CPE |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in ICC/IF was reported in scientific literature (PMID: 28978054). Immunohistochemistry-Frozen from a verified customer review. |
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| Theoretical MW | 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-15699 | Applications | Species |
|---|---|---|
| Ilina Elena I, Armento Angela, Sanchez Leticia Garea et al. Effects of soluble CPE on glioma cell migration are associated with mTOR activation and enhanced glucose flux. Oncotarget 2017-06-27 [PMID: 28978054] (ICC, Human) | ICC | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Parisa Shabani |
IHC-Fr | Rat | 06/27/2022 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Representative dual-immunofluorescence staining of rat endothelial cell antigen-1 (RECA-1) and carboxypeptidase E (CPE) in rat heart frozen sections. Image from verified customer review.](http://images.novusbio.com/fullsize2/Carboxypeptidase-E-CPE-Antibody-Immunohistochemistry-Frozen-NBP2-15699-img0008.jpg "Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Representative dual-immunofluorescence staining of rat endothelial cell antigen-1 (RECA-1) and carboxypeptidase E (CPE) in rat heart frozen sections. Image from verified customer review.")
![Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize2/Carboxypeptidase-E-CPE-Antibody-Western-Blot-NBP2-15699-img0007.jpg "Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Human breast cancer. Carboxypeptidase E antibody [N2C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize2/Carboxypeptidase-E-CPE-Antibody-Immunohistochemistry-Paraffin-NBP2-15699-img0004.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Human breast cancer. Carboxypeptidase E antibody [N2C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
