Carboxypeptidase B2/CPB2 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Carboxypeptidase B2/CPB2 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B2/CPB2
  Phe23-Val423 (Ala169Thr)
  Accession # Q96IY4
• Specificity: Detects Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CPA1, rhCPB1 and recombinant mouse CPB1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase B2/CPB2 Antibody [Alexa Fluor® 750]

• carboxypeptidase B2 (plasma)
• carboxypeptidase B2 (plasma, carboxypeptidase U)
• Carboxypeptidase B2
• Carboxypeptidase U
• CPB2
• CPU
• CPUEC 3.4.17.20
• EC 3.4.17
• PCPB
• Plasma carboxypeptidase B
• TAFI
• TAFIcarboxypeptidase B-like protein
• Thrombin-activable fibrinolysis inhibitor
• thrombin-activatable fibrinolysis inhibitor

Background

CPB2 (Carboxypeptidase B2; also CPU and TAFI) is a secreted, 50-60 kDa glycoprotein member of the peptidase M14 family of enzymes. It is expressed by hepatocytes (60 kDa) and platelets (50 kDa), with MW differences attributable to glycosylation. CPB2 is cleaved by thrombin and plasmin, generating a 36 kDa, relatively insoluble nonglycosylated enzymatically active fragment (TAFIa). Active CPB2 removes C-terminal Lys residues from fibrin, thereby interrupting plasmin generation and promoting fibrin polymerization. Human CPB2 (proprecursor/zymogen) is 401 amino acids (aa) in length. It contains a prosequence (aa 23-114) and an active fragment (aa 115-423) that acts on C-terminal Lys or Arg residues. There is one potential isoform variant that shows a deletion of aa 198-234 accompanied by a 16 aa substitution for aa 382-423. Over aa 23-423, human CPB2 shares 85% aa identity with mouse CPB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.