Carboxypeptidase B1/CPB1 Antibody (4D5)

Images

 
Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Analysis of CPB1 expression in transfected 293T cell line by CPB1 monoclonal antibody (M01), clone 4D5.Lane 1: CPB1 transfected lysate(47.4 ...read more
Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Analysis of monoclonal antibody to CPB1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Detection against Immunogen (70.55 KDa)
Immunoprecipitation: Carboxypeptidase B1/CPB1 Antibody (4D5) [H00001360-M01] - Analysis of CPB1 transfected lysate using anti-CPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CPB1 MaxPab ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P, IP
Clone
4D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Carboxypeptidase B1/CPB1 Antibody (4D5) Summary

Immunogen
CPB1 (AAH15338, 14 a.a. - 417 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SAHHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY
Specificity
carboxypeptidase B1 (tissue)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CPB1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Carboxypeptidase B1/CPB1 Antibody (4D5)

  • carboxypeptidase B
  • carboxypeptidase B1 (tissue)
  • Carboxypeptidase B1
  • CPB
  • CPB1
  • DKFZp779K1333
  • EC 3.4.17
  • EC 3.4.17.2
  • Pancreas-specific protein
  • PASP
  • PCPB
  • procarboxypeptidase B

Background

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Carboxypeptidase B1/CPB1 Antibody (H00001360-M01). (Showing 1 - 2 of 2 FAQ).

  1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
    • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
  2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.
    • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CPB1
Entrez
OMIM
Uniprot