Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) - Azide and BSA Free

Western Blot: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) - Azide and BSA Free [NBP2-79880] - Western Blot Analysis of pancreatic tissue lysate using CPA1 Mouse Monoclonal Antibody (CPA1/2712).

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) - Azide and BSA Free [NBP2-79880] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1 / CPA1 Mouse Monoclonal Antibody (CPA1/2712).

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) - Azide and BSA Free [NBP2-79880] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1 / CPA1 Mouse Monoclonal Antibody (CPA1/2712).

Protein Array: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) - Azide and BSA Free [NBP2-79880] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

• Reactivity: Hu, Ca
• Applications: WB, IHC, MA
• Clone: CPA1/2712
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79745).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human Carboxypeptidase A1/CPA1 protein (Uniprot: P15085)
• Localization: Secreted
• Marker: Pancreatic Cancer Marker
• Isotype: IgG2c Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CPA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) - Azide and BSA Free

• carboxypeptidase A1 (pancreatic)
• Carboxypeptidase A1
• CPA
• CPA1
• EC 3.4.17
• EC 3.4.17.1

Background

Carboxypeptidase A is a pancreatic exopeptidase. Three different forms of human pancreatic procarboxypeptidase A have been isolated. The A1 and A2 forms are monomeric proteins with different biochemical properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPA1