![Western Blot: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Staining of human tonsil section. Image using the Alexa Fluor 488 format of this antibody.](http://images.novusbio.com/fullsize2/Carboxypeptidase-A1-CPA1-Antibody-(CPA1-2712)-Western-Blot-NBP2-79745-img0004.jpg "Western Blot: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Staining of human tonsil section. Image using the Alexa Fluor 488 format of this antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IHC-P, PA |
| Clone | CPA1/2712 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Recombinant full-length human Carboxypeptidase A1/CPA1 protein |
| Localization | Secreted |
| Isotype | IgG2c Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CPA1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Western Blot: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Western-Blot-NBP2-73857-img0001.jpg)
![Flow Cytometry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RFC2 antibody.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-73857-img0002.jpg)
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.](http://images.novusbio.com/images2/RFC4-Antibody-(OTI1A8)-Immunofluorescence-NBP2-45946-img0001.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/RFC4-Antibody-(OTI1A8)-Immunohistochemistry-NBP2-45946-img0002.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/RFC4-Antibody-(OTI1A8)-Immunohistochemistry-NBP2-45946-img0003.jpg)
![Flow Cytometry: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (PA1041A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/images2/Adenosine-A1-R-Antibody-Flow-Cytometry-NB300-549-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells.](http://images.novusbio.com/images2/Adenosine-A1-R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-549-img0007.jpg)
![Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).](http://images.novusbio.com/images2/Adenosine-A1-R-Antibody-Western-Blot-NB300-549-img0008.jpg)
![Western Blot: GPHA2 Antibody [NBP3-17255] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/images2/GPHA2 Antibody-Western Blot-NBP3-17255-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GPHA2 Antibody [NBP3-17255] - Staining of human cell line Hep G2 shows localization to vesicles.](http://images.novusbio.com/images2/GPHA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17255-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A2 Antibody [NBP1-59069] - overexpressed mouse sequences tissue](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Immunohistochemistry-Paraffin-NBP1-59069-img0005.jpg)
![Flow Cytometry: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of Hela cells with gamma Synuclein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/images2/gamma-Synuclein-Antibody-(JM90-32)-Flow-Cytometry-NBP2-66861-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Staining gamma Synuclein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/gamma-Synuclein-Antibody-(JM90-32)-Immunocytochemistry-Immunofluorescence-NBP2-66861-img0002.jpg)
![Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of paraffin-embedded human breast cancer tissue using anti-gamma Synuclein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/gamma-Synuclein-Antibody-(JM90-32)-Immunohistochemistry-Paraffin-NBP2-66861-img0003.jpg)
![Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Carboxypeptidase-B1-CPB1-Antibody-Western-Blot-NBP2-15698-img0002.jpg)
![Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Carboxypeptidase-B1-CPB1-Antibody-Western-Blot-NBP2-15698-img0004.jpg)
![Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Carboxypeptidase-B1-CPB1-Antibody-Western-Blot-NBP2-15698-img0006.jpg)
![Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/Adenosine-A2aR-Antibody-(7F6-G5-A2)-Western-Blot-NBP1-39474-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Adenosine-A2aR-Antibody-(7F6-G5-A2)-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Adenosine-A2aR-Antibody-(7F6-G5-A2)-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0005.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0010.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0011.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Analysis in human adrenal gland and liver tissues. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0012.jpg)
![SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005555-P01-1.jpg)
![Flow Cytometry: Carboxypeptidase A2 Antibody (1C7) [NBP2-01992] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Carboxypeptidase A2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images/Carboxypeptidase A2 Antibody (1C7)-Flow Cytometry-NBP2-01992-img0001.jpg)
![Western Blot: Carboxypeptidase A2 Antibody (1C7) [NBP2-01992] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Carboxypeptidase A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Carboxypeptidase A2.](http://images.novusbio.com/images/Carboxypeptidase A2 Antibody (1C7)-Western Blot-NBP2-01992-img0002.jpg)
![Immunohistochemistry-Paraffin: Carboxypeptidase A2 Antibody (1C7) [NBP2-01992] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Carboxypeptidase A2 mouse monoclonal antibody.](http://images.novusbio.com/images/Carboxypeptidase A2 Antibody (1C7)-Immunohistochemistry-Paraffin-NBP2-01992-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Carboxypeptidase A1/CPA1 Antibody (NBP2-79745)Discover more about diseases related to Carboxypeptidase A1/CPA1 Antibody (NBP2-79745).
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![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Antibody (CPA1/2712).](http://images.novusbio.com/fullsize2/Carboxypeptidase-A1-CPA1-Antibody-(CPA1-2712)-Immunohistochemistry-Paraffin-NBP2-79745-img0002.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Antibody (CPA1/2712).")
![Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Antibody (CPA1/2712).](http://images.novusbio.com/fullsize2/Carboxypeptidase-A1-CPA1-Antibody-(CPA1-2712)-Immunohistochemistry-Paraffin-NBP2-79745-img0001.jpg "Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Antibody (CPA1/2712).")
![Protein Array: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Carboxypeptidase A1/CPA1 Antibody (CPA1/2712). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize2/Carboxypeptidase-A1-CPA1-Antibody-(CPA1-2712)-Protein-Array-NBP2-79745-img0003.jpg "Protein Array: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Carboxypeptidase A1/CPA1 Antibody (CPA1/2712). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
![Immunocytochemistry/Immunofluorescence: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/ATP6V0A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89342-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/images2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0011.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human kidney shows strong cytoplasmic postivity in cells in tubules.](http://images.novusbio.com/images2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0002.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/images2/Androgen-R-NR3C4-[p-Ser213--p-Ser210]-Antibody-(156C135.2)-Western-Blot-NB100-56603-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0016.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/Adenosine-Deaminase-ADA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87404-img0013.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).](http://images.novusbio.com/images2/Thyroglobulin-Antibody-(2H11-+-6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0003.jpg)
![Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.](http://images.novusbio.com/images2/Thyroglobulin-Antibody-(2H11-+-6E1)-Immunohistochemistry-NBP2-34294-img0004.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/images2/Thyroglobulin-Antibody-(2H11-+-6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0005.jpg)
