Carbonic Anhydrase VIII/CA8 Antibody (1F7)

Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (1F7) [H00000767-M01] - CA8 monoclonal antibody (M01), clone 1F7 Analysis of CA8 expression in A-549.

Sandwich ELISA: Carbonic Anhydrase VIII/CA8 Antibody (1F7) [H00000767-M01] - Detection limit for recombinant GST tagged CA8 is approximately 3ng/ml as a capture antibody.

Product Discontinued

Carbonic Anhydrase VIII/CA8 Antibody (1F7) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CA8 (NP_004047, 40 a.a. ~ 139 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VFPDANGEYQSPINLNSREARYDPSLLDVRLSPNYVVCRDCEVTNDGHTIQVILKSKSVLSGGPLPQGHEFELYEVRFHWGRENQRGSEHTVNFKAFPME
• Specificity: CA8 - carbonic anhydrase VIII
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CA8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Carbonic Anhydrase VIII/CA8 Antibody (1F7)

• CA8
• CALS
• CALScarbonate dehydratase
• CAMRQ3
• Carbonic Anhydrase VIII
• carbonic anhydrase VIIIMGC99509
• carbonic anhydrase-like sequence
• carbonic anhydrase-related protein
• CARP
• CARPCA-related protein
• CA-VIII
• MGC120502

Background

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA8
• Entrez:
  • 767(Human)
• OMIM:
  • 114815(Human)
• Uniprot:
  • NP_004047(Human)