Carbonic Anhydrase VA/VB (CA5A/5B) Antibody (487908)

Carbonic Anhydrase VA/VB (CA5A/5B) was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Carbonic Anhydrase VA/ VB (CA5A/5B) Monoclonal Antibody (Catalog # MAB5388) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: 487908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase VA/VB (CA5A/5B) Antibody (487908) Summary

• Immunogen: E. coli-derived recombinant human Carbonic Anhydrase VA/VB (CA5A/5B)
  Ala40-Ser305
  Accession # P35218
• Specificity: Detects human Carbonic Anhydrase VA/VB (CA5A/5B) in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6‑14 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CA5A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase VA/VB (CA5A/5B) Antibody (487908)

• CA5;CA5AD;Carbonic anhydrase 5A, mitochondrial;CAV;CAVA;GS1-21A4.1
• Carbonic Anhydrase VA/VB

Background

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O → HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA5A and CA5B are homologous proteins that are encoded by different genes locating in different chromosomes. However, they both are mitochondrial proteins and have low Carbonic Anhydrase activity. The amino acid sequence of human CA5A is 98%, 80% and 76% identical to that of chimpanzee, dog and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA5A
• Uniprot:
  • P35218()