Recombinant Human Carbonic Anhydrase II/CA2 Protein

SDS-Page: Recombinant Human Carbonic Anhydrase II/CA2 Protein [NBC1-28002] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Discontinued

Recombinant Human Carbonic Anhydrase II/CA2 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-260 of Human Carbonic Anhydrase II/CA2
  
  

  Source: E.coli

  Amino Acid Sequence: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

• Details of Functionality: Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CA2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 29.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate-Buffered Saline (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Carbonic Anhydrase II/CA2 Protein

• CA2
• CAII
• CA-IIEC 4.2.1.1
• Car2
• Carbonate dehydratase II
• carbonic anhydrase 2
• carbonic anhydrase B
• Carbonic anhydrase C
• Carbonic Anhydrase II
• carbonic anhydrase IICAC
• carbonic dehydratase

Background

Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CA2