Carbohydrate Sulfotransferase 15/CHST15 Antibody

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbohydrate Sulfotransferase 15/CHST15 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carbohydrate Sulfotransferase 15/CHST15
  Ser99-Thr561
  Accession # Q7LFX5
• Specificity: Detects human Carbohydrate Sulfotransferase 15/CHST15 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHST15
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 15/CHST15 Antibody

• B cell RAG associated protein (GALNAC4S-6ST)
• B-cell RAG-associated gene protein
• BRAG
• BRAGKIAA0598RP11-47G11.1
• carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
• Carbohydrate Sulfotransferase 15
• CHST15
• DKFZp781H1369
• EC 2.8.2.33
• GALNAC4S6ST
• GalNAc4S-6ST
• hBRAG
• MGC34346
• N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase

Background

N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST, or, CHST15) is a type II transmembrane protein composed of a short N-terminal cytoplasmic domain and a long C-terminal luminal catalytic domain. GalNAc4S-6ST transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the 6-O of GalNAc-4S on chondroitin sulfate A (CS-A), as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS-E) disaccharide unit GlcA beta 1-3GalNAc-4S,6S. CS-E binds with strong affinity to Midkine, a heparan sulfate-binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of GalNAc4S-6ST in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6-O sulfotransferase-1 (C6ST-1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6-O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS-C) disaccharide unit GlcA beta 1-3GalNAc-6S (4). The sequence of the human GalNAc4S-6ST was found to be the same as human B-cell RAG-associated protein (BRAG) (1).

1. Ohtake, S. et al. (2001) J. Biol. Chem. 276:43894.
2. Zou, P. et al. (2003) Glycobiology 13:35.
3. Purushothaman, A. et al. (2007) J. Biol. Chem. 282:19442.
4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10155.
5. Robbins, P.W. (1962) Methods in Enzymol. 5:964.
6. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
7. Wu, Z.L. et al. (2002) FASEB J. 16:539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbohydrate Sulfotransferase 15/CHST15 Antibody (AF3365)(1)

Publication using AF3365

• Zensho Ito, Kazuki Takakura, Machi Suka, Tomoya Kanai, Ryota Saito, Shuichi Fujioka et al. Prognostic impact of carbohydrate sulfotransferase 15 in patients with pancreatic ductal adenocarcinoma Oncology Letters 2017-06-01 [PMID: 28599481]

Bioinformatics

• Gene Symbol: CHST15
• Uniprot:
  • Q7LFX5()