Carbohydrate Sulfotransferase 15/CHST15 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

Carbohydrate Sulfotransferase 15/CHST15 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carbohydrate Sulfotransferase 15/CHST15
  Ser99-Thr561
  Accession # Q7LFX5
• Specificity: Detects human Carbohydrate Sulfotransferase 15/CHST15 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 15/CHST15 Antibody [Alexa Fluor® 750]

• B cell RAG associated protein (GALNAC4S-6ST)
• B-cell RAG-associated gene protein
• BRAG
• BRAGKIAA0598RP11-47G11.1
• carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
• Carbohydrate Sulfotransferase 15
• CHST15
• DKFZp781H1369
• EC 2.8.2.33
• GALNAC4S6ST
• GalNAc4S-6ST
• hBRAG
• MGC34346
• N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase

Background

N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST, or, CHST15) is a type II transmembrane protein composed of a short N-terminal cytoplasmic domain and a long C-terminal luminal catalytic domain. GalNAc4S-6ST transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the 6-O of GalNAc-4S on chondroitin sulfate A (CS-A), as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS-E) disaccharide unit GlcA beta 1-3GalNAc-4S,6S. CS-E binds with strong affinity to Midkine, a heparan sulfate-binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of GalNAc4S-6ST in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6-O sulfotransferase-1 (C6ST-1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6-O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS-C) disaccharide unit GlcA beta 1-3GalNAc-6S (4). The sequence of the human GalNAc4S-6ST was found to be the same as human B-cell RAG-associated protein (BRAG) (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.