Carbohydrate Sulfotransferase 15/CHST15 Antibody (388421) [Alexa Fluor® 700]

• Applications: WB
• Clone: 388421
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Carbohydrate Sulfotransferase 15/CHST15 Antibody (388421) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Sulfotransferase 15/CHST15
  Ser99-Thr561
  Accession # Q7LFX5
• Specificity: Detects human Carbohydrate Sulfotransferase 15/CHST15 in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST or CHST15) is a type II transmembrane protein composed of a short N‑terminal cytoplasmic domain and a long C-terminal luminal catalytic domain. CHST15 transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the 6-O of GalNAc-4S on chondroitin sulfate A (CS-A) as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS-E) disaccharide unit GlcA beta 1-3GalNAc-4S,6S. CS-E binds with strong affinity to Midkine, a heparan sulfate-binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of CHST15 in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6-O sulfotransferase-1 (C6ST-1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6-O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS-C) disaccharide unit GlcA beta 1-3GalNAc-6S (4). The sequence of the human CHST15 was found to be the same as human B-cell RAG-associated protein (BRAG) (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.