CAPZB Antibody

Western Blot: CAPZB Antibody [NB100-814] - (1ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane).

Western Blot: CAPZB Antibody [NB100-814] - Analysis of of Jurkat (A), HeLa (B) and NIH-3T3 (C) lysates (35ug protein in RIPA buffer).

Western Blot: CAPZB Antibody [NB100-814] - Analysis of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CAPZB Antibody Summary

• Immunogen: Peptide with sequence NDLVEALKRKQQC corresponding to C-Terminus according to NP_004921.1.
• Specificity: This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form.
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CAPZB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in Jurkat, HeLa and NIH-3T3 lysates and approx. 28 kDa band observed in mouse, rat and porcine heart lysates (calculated MW of 30.6 kDa band according to human NP_004921.1 and 31.3 kDa band according to mouse NP_001032850.1, and 30.6 kDa band according to rat NP_001005903.1 and porcine NP_001106915.1].
• Control:

  Jurkat Whole Cell Lysate
  HeLa Whole Cell Lysate
  CAPZB Overexpression Lysate
  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CAPZB Antibody

• Cap Z
• CAPB
• CAPPB
• capping protein (actin filament) muscle Z-line, beta
• CapZ beta
• CAPZ
• F-actin capping protein beta subunit
• F-actin-capping protein subunit beta
• MGC104401
• MGC129749
• MGC129750

Background

CAPZB is involved in the regulation of growth of the actin filament by capping the barbed end of growing actin filaments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CAPZB Antibody (NB100-814)(1)

Publication using NB100-814

• Hart MC, Cooper JA. Vertebrate isoforms of actin capping protein beta have distinct functions In vivo.gelsolin/severin and profilin protein families. J Cell Biol 1999-12-13 [PMID: 10601341]

Bioinformatics

• Gene Symbol: CAPZB
• Entrez:
  • 832(Human)
  • 12345(Mouse)
• Uniprot:
  • NP_004921.1(Human)