Human Cannabinoid R1/CB1/CNR1 ELISA Kit (Chemiluminescence) Summary
| Specificity |
This kit recognizes Human CNR1 in samples. No significant cross-reactivity or interference between Human CNR1 and analogues was observed. |
| Standard Curve Range |
31.25-2000 pg/mL |
| Sensitivity |
18.75 pg/mL |
| Assay Type |
Sandwich-CLIA |
| Inter-Assay |
CV% < 9.67% |
| Intra-Assay |
CV% < 10.08% |
| Spike Recovery |
93-113% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Chemiluminescence) |
| Gene |
CNR1 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Human Cannabinoid R1/CB1/CNR1 ELISA Kit (Chemiluminescence)
Background
CB1 Cannabinoid receptor is associated with the regulation of cognition, memory, and motor activity. The CB1 receptor mediates cannabinoid-induced CNS effects and the addictive behavior experienced by users of marijuana. Three splice variants that are produced by alternative splicing have been identified for this gene. CB1 has been reported primarily in the brain and, to a lower extent, in peripheral tissues including adrenal, heart, lung, prostate, uterus, ovary, testis, bone marrow, thymus, and tonsil. ESTs have been isolated from brain, embryo, heart/melanocyte/uterus, lung, placenta, testis, tonsil, and vessel libraries.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Cannabinoid R1/CB1/CNR1 ELISA Kit (NBP2-75792). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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