Canine IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate

• Reactivity: Ca
• Applications: ELISA
• Conjugate: Biotin

Canine IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL1B

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate

• Catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Publications for IL-1 beta/IL-1F2 (DY3747)(8)

Publications using DY3747

• Jung, K;Ku, JY;Kwon, JS;Won, G;Yoon, H;Oh, SI;Kim, MH;Kim, C;Yoon, JS; IFN-?/TNF-? Synergism Induces Pro-Inflammatory Cytokine and Chemokine Production by In Vitro Canine Keratinocytes Veterinary sciences 2025-01-14 [PMID: 39852930] (Canine)
• Costa, SF;Soares, MF;Poleto Bragato, J;Dos Santos, MO;Rebech, GT;de Freitas, JH;de Lima, VMF; MicroRNA-194 regulates parasitic load and IL-1?-dependent nitric oxide production in the peripheral blood mononuclear cells of dogs with leishmaniasis PLoS neglected tropical diseases 2024-01-01 [PMID: 38241360] (Canine)
• GT Rebech, JP Bragato, SF Costa, JH de Freitas, MO Dos Santos, MF Soares, FR Eugênio, PSP Dos Santos, VMF de Lima miR-148a regulation interferes in inflammatory cytokine and parasitic load in canine leishmaniasis PloS Neglected Tropical Diseases, 2023-01-31;17(1):e0011039. 2023-01-31 [PMID: 36719867] (Canine)
• S Kim, YS Hyun, HT Park, MK Shin, HS Yoo Mycobacterium intracellulare induces a Th17 immune response via M1-like macrophage polarization in canine peripheral blood mononuclear cells Scientific Reports, 2022-07-12;12(1):11818. 2022-07-12 [PMID: 35821058] (Canine)
• JC Alves, A Santos, P Jorge, C Lavrador, LM Carreira Effect of a single intra-articular administration of stanozolol in a naturally occurring canine osteoarthritis model: a randomised trial Scientific Reports, 2022-04-07;12(1):5887. 2022-04-07 [PMID: 35393497] (Canine)
• Z Digby, P Tourlomous, J Rooney, JP Boyle, B Bibo-Verdu, RJ Pickering, SJ Webster, TP Monie, LJ Hopkins, N Kayagaki, GS Salvesen, S Warming, L Weinert, CE Bryant Evolutionary loss of inflammasomes in the Carnivora and implications for the carriage of zoonotic infections Cell Reports, 2021-08-24;36(8):109614. 2021-08-24 [PMID: 34433041] (Canine)
• JC Alves, A Santos, P Jorge, C Lavrador, LM Carreira The intra-articular administration of triamcinolone hexacetonide in the treatment of osteoarthritis. Its effects in a naturally occurring canine osteoarthritis model PLoS ONE, 2021-01-20;16(1):e0245553. 2021-01-20 [PMID: 33471857] (Canine)
• T Ichiki, BK Huntley, GJ Harty, SJ Sangaralin, JC Burnett Early activation of deleterious molecular pathways in the kidney in experimental heart failure with atrial remodeling Physiol Rep, 2017-05-15;5(9):. 2017-05-15 [PMID: 28507167] (Canine)

FAQs for IL-1 beta/IL-1F2 (DY3747). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL1B