Human CAMP/Cathelicidin ELISA Kit (Colorimetric)

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Human CAMP/Cathelicidin ELISA Kit (Colorimetric) [NBP3-21152] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
0.45 ng/mL - 30 ng/mL
Sensitivity
0.11 ng/mL

Order Details

Human CAMP/Cathelicidin ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human CAMP/Cathelicidin. No significant cross-reactivity or interference between human CAMP/Cathelicidin and analogues was observed.
Standard Curve Range
0.45 ng/mL - 30 ng/mL
Sensitivity
0.11 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Spike Recovery
89 - 100%
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CAMP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human CAMP/Cathelicidin ELISA Kit (Colorimetric)

  • 18 kDa cationic antimicrobial protein
  • Antibacterial peptide FALL-39
  • CAP18
  • CAP-18
  • cathelicidin antimicrobial peptide
  • CRAMP
  • epididymis secretory sperm binding protein
  • FALL-39 peptide antibiotic
  • FALL39
  • FALL-39
  • hCAP-181
  • HSD26
  • LL37

Background

Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase negative granules of neutrophils. The cathelicidins are synthesized as preproproteins. Within the neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide. The active biologic domains of the cathelicidins generally reside in the C terminus. The C terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophil granules. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CAMP