CaMKK2 Antibody (1A11) - Azide and BSA Free

Western Blot: CaMKK2 Antibody (1A11) [H00010645-M01] - CAMKK2 monoclonal antibody (M01), clone 1A11. Analysis of CAMKK2 expression in K-562.

Western Blot: CaMKK2 Antibody (1A11) [H00010645-M01] - CAMKK2 monoclonal antibody (M01), clone 1A11 Analysis of CAMKK2 expression in IMR-32.

Western Blot: CaMKK2 Antibody (1A11) [H00010645-M01] - Analysis of CAMKK2 expression in transfected 293T cell line by CAMKK2 monoclonal antibody (M01), clone 1A11.Lane 1: CAMKK2 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: CaMKK2 Antibody (1A11) [H00010645-M01] - Analysis of CAMKK2 transfected lysate using anti-CAMKK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CAMKK2 MaxPab rabbit polyclonal antibody.

ELISA: CaMKK2 Antibody (1A11) [H00010645-M01] - Detection limit for recombinant GST tagged CAMKK2 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clone: 1A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CaMKK2 Antibody (1A11) - Azide and BSA Free Summary

• Immunogen: CAMKK2 (AAH26060, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSCVSSQPSSNRAAPQDELGGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLARDRPLEADGQEVPLDSSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICPSLPYSP
• Specificity: CAMKK2 - calcium/calmodulin-dependent protein kinase kinase 2, beta
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAMKK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25366347)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CaMKK2 Antibody (1A11) - Azide and BSA Free

• calcium/calmodulin-dependent protein kinase kinase 2
• calcium/calmodulin-dependent protein kinase kinase 2, beta
• Calcium/calmodulin-dependent protein kinase kinase beta
• CaM-kinase kinase 2
• CaM-kinase kinase beta
• CaMKK 2
• CaM-KK 2
• CAMKK beta protein
• CaMKK beta
• CaM-KK beta
• CAMKK
• CaMKK2
• CAMKKB
• CAMKKBCaMKK 2
• EC 2.7.11
• EC 2.7.11.17
• KIAA0787
• KIAA0787calcium/calmodulin-dependent protein kinase beta
• MGC15254

Background

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaMKK2 Antibody (H00010645-M01)(6)

Publications using H00010645-M01

• Angela G, Shuhang D, Arthur E et al. Distinguishing parallel from series circuits with a double knockdown procedure in human cell lines. STAR Protoc. 2022-12-16 [PMID: 36595936]
• Dai S, Venturini E, Yadav S et al. Calcium/calmodulin-dependent protein kinase kinase 2 mediates pleiotropic effects of epidermal growth factor in cancer cells Biochimica et biophysica acta. Molecular cell research 2022-03-07 [PMID: 35271909] (WB, Human)
• Kim BM, Kim DH, Ha S et al. PAR2 promotes high-fat diet-induced hepatic steatosis by inhibiting AMPK-mediated autophagy The Journal of nutritional biochemistry 2021-05-14 [PMID: 34000413] (WB, Mouse)
• Truve K, Dickinson P, Xiong A et al. Utilizing the Dog Genome in the Search for Novel Candidate Genes Involved in Glioma Development-Genome Wide Association Mapping followed by Targeted Massive Parallel Sequencing Identifies a Strongly Associated Locus. PLoS Genet 2016-05-12 [PMID: 27171399]
• Morrison A, Chen L, Wang J. Sestrin2 promotes LKB1-mediated AMPK activation in the ischemic heart FASEB J. 2014-11-03 [PMID: 25366347] (WB, Mouse)
  
  Details:
  CaMKK2 / Ca2+ calmodulin dependent protein kinase beta antibody used for WB on Sestrin2 immune-complexes from heart, and on Brain tissue (positive control) from Male wild-type and Sesn2 KO mice (Supplemental Figure 2).
• Karacosta LG, Foster BA, Azabdaftari G et al. A regulatory feedback loop between Ca2+/Calmodulin-dependent protein kinase kinase 2 (CaMKK2) and the androgen receptor in prostate cancer progression. J Biol Chem. 2012-05-31 [PMID: 22654108]

Bioinformatics

• Gene Symbol: CAMKK2
• Entrez:
  • 10645(Human)
• Uniprot:
  • AAH26060(Human)