CaMKIV Antibody (JB33-13)

Western Blot: CaMKIV Antibody (JB33-13) [NBP2-76979] - Western blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (JB33-13) [NBP2-76979] - ICC staining CAMKIV in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CaMKIV Antibody (JB33-13) [NBP2-76979] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CaMKIV antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: CaMKIV Antibody (JB33-13) [NBP2-76979] - Flow cytometric analysis of SH-SY-5Y cells with CAMKIV antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (JB33-13) [NBP2-76979] - ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (JB33-13) [NBP2-76979] - ICC staining CAMKIV in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CaMKIV Antibody (JB33-13) [NBP2-76979] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.

Immunohistochemistry: CaMKIV Antibody (JB33-13) [NBP2-76979] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.

Immunohistochemistry: CaMKIV Antibody (JB33-13) [NBP2-76979] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.

Product Discontinued

CaMKIV Antibody (JB33-13) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CaMKIV aa 360-473 / 473. (SwissProt: Q16566 Human; SwissProt: P08414 Mouse; SwissProt: P13234 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAMK4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CaMKIV Antibody (JB33-13)

• brain Ca(2+)-calmodulin-dependent protein kinase type IV
• brain Ca++-calmodulin-dependent protein kinase type IV
• calcium/calmodulin-dependent protein kinase IV
• calcium/calmodulin-dependent protein kinase type IV catalytic chain
• calcium/calmodulin-dependent protein kinase type IV
• CAM kinase- GR
• CAM kinase IV
• CaM kinase-GR
• CaMK IV
• caMK
• CaMK-GR
• EC 2.7.11
• EC 2.7.11.17
• IV
• MGC36771

Background

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK4