CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) - BSA Free

Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII alpha/beta protein using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:1000. Analysis of CaMKII alpha/beta and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.

Immunocytochemistry/Immunofluorescence: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.

Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.

Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 22B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) - BSA Free Summary

• Immunogen: Synthetic peptide
• Modification: p Thr286, p Thr287
• Localization: Cytoplasm , Mitochondrion , Nucleus , Cell Junction , Synapse , Presynaptic Cell Membrane
• Specificity: This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAMK2A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: 1 ug/ml was sufficient for detection of 0.2 ug CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Use in immunoblotting reported in scientific literature (PMID: 27599291).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) - BSA Free

• calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• calcium/calmodulin-dependent protein kinase II alpha
• calcium/calmodulin-dependent protein kinase II alpha-B subunit
• CaM kinase II alpha subunit
• CaM kinase II subunit alpha
• CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
• CaMK-II alpha subunit
• CaMK-II subunit alpha
• CaMKIINalpha
• CaM-kinase II alpha chain
• EC 2.7.11
• EC 2.7.11.17
• KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho- CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Auto-phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaMKII alpha/beta Antibody (NB110-96869)(8)

Publications using NB110-96869

• Kim Y, Jang E, Lee Y et al. Miniature Transparent Dopamine Sensor Based on Nanosphere Lithography Advanced Materials Technologies 2023-04-02 (WB)
• LaMoia TE, Hubbard BT, Guerra MT, Nasiri A et Al. Cytosolic calcium regulates hepatic mitochondrial oxidation, intrahepatic lipolysis, and gluconeogenesis via CAMKII activation Cell Metab 2024-08-17 [PMID: 39153480]
• Ghane MA, Wei W, Yakout DW et al. Arc ubiquitination regulates endoplasmic reticulum-mediated Ca2+ release and CaMKII signaling Frontiers in cellular neuroscience 2023-03-14 [PMID: 36998269] (Western Blot, Mouse)
• Lee Y, Kang JS, Ham OJ et al. Gut metabolite trimethylamine N-oxide induces aging-associated phenotype of midbrain organoids for the induced pluripotent stem cell-based modeling of late-onset disease Frontiers in Aging Neuroscience 2022-08-16 [PMID: 36051303] (Western Blot, Mouse)
• Cai B, Kasikara C, Doran AC et al. MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity. Sci Signal 2018-09-25 [PMID: 30254055] (WB, Mouse)
• Ersoy BA, Maner-Smith KM, Li Y et al. Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress. J. Clin. Invest. 2017-11-20 [PMID: 29202465] (WB, Mouse)
• Conlon DM, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J Clin Invest 2016-09-06 [PMID: 27599291] (IB)
• Jiang L, Xu L, Song Y et al. CaMK II/CREB/Wnt/beta-catenin signaling cascade regulates Angiotensin II-induced podocyte injury and albuminuria. J Biol Chem 2013-06-26 [PMID: 23803607] (WB, Mouse)

Bioinformatics

• Gene Symbol: CAMK2A
• Entrez:
  • 815(Human)
  • 25400(Rat)
• Uniprot:
  • P11275(Rat)