CaM Kinase II alpha [p Thr286] Antibody

Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NBP3-13299] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II alpha (phospho Thr286) antibody (NBP3-13299) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Mu, Hu, Rt, Bv, Ch
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CaM Kinase II alpha [p Thr286] Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Thr286 of human CaM Kinase II alpha. The exact sequence is proprietary.
• Modification: p Thr286
• Predicted Species: Human (100%), Rat (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAMK2A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CaM Kinase II alpha [p Thr286] Antibody

• calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• calcium/calmodulin-dependent protein kinase II alpha
• calcium/calmodulin-dependent protein kinase II alpha-B subunit
• CaM kinase II alpha subunit
• CaM Kinase II alpha
• CaM kinase II subunit alpha
• CAMK2A
• CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
• CaMK-II alpha subunit
• CaMK-II subunit alpha
• CaMKIINalpha
• CaM-kinase II alpha chain
• EC 2.7.11
• EC 2.7.11.17
• KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK2A