CaM Kinase I Antibody

Images

 
Western Blot: CaMKI Antibody [NBP2-15683] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CaMKI Antibody [NBP2-15683] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.78 mg/ml

Order Details

CaM Kinase I Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CaMK1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (93%), Xenopus tropicalis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.78 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CaM Kinase I Antibody

  • calcium/calmodulin-dependent protein kinase I
  • calcium/calmodulin-dependent protein kinase type 1
  • CaM kinase I alpha
  • CaM Kinase I
  • CAMKI
  • caM-KI
  • caMKI-alpha
  • EC 2.7.11
  • EC 2.7.11.17
  • MGC120317
  • MGC120318

Background

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CAMK1
Uniprot