CaM Kinase I Antibody

Western Blot: CaMKI Antibody [NBP2-15683] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: CaMKI Antibody [NBP2-15683] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: CaM Kinase I Antibody [NBP2-15683] - Paraffin-embedded mouse kidney. CaMK1 antibody [N2C3] dilution: 1:500

Product Discontinued

CaM Kinase I Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CaMK1. The exact sequence is proprietary.
• Predicted Species: Rat (100%), Porcine (99%), Bovine (99%), Rhesus Macaque (100%), Xenopus (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAMK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (93%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CaM Kinase I Antibody

• calcium/calmodulin-dependent protein kinase I
• calcium/calmodulin-dependent protein kinase type 1
• CaM kinase I alpha
• CaM Kinase I
• CAMKI
• caM-KI
• caMKI-alpha
• EC 2.7.11
• EC 2.7.11.17
• MGC120317
• MGC120318

Background

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CAMK1
• Uniprot:
  • Q14012()