Calsequestrin 1 Antibody (5P6U2)

Western Blot: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831] - Western blot analysis of extracts of Rat heart, using Calsequestrin 1 Rabbit mAb (NBP3-15831) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Western Blot: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831] - Western blot analysis of Mouse skeletal muscle, using Calsequestrin 1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.

Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831] - Immunofluorescence analysis of Rat skeletal muscle using Calsequestrin 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831] - Immunofluorescence analysis of Mouse skeletal muscle using Calsequestrin 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 5P6U2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Calsequestrin 1 Antibody (5P6U2) Summary

• Description: Novus Biologicals Rabbit Calsequestrin 1 Antibody (5P6U2) (NBP3-15831) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_001222.3). EDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDA
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CASQ1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Calsequestrin 1 Antibody (5P6U2)

• calmitin
• calmitine
• calsequestrin 1 (fast-twitch, skeletal muscle)
• Calsequestrin, skeletal muscle isoform
• calsequestrin-1
• CASQ
• PDIB1

Background

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASQ1